Journal: Micromachines

Article Title: Glioma-on-a-Chip Models

doi: 10.3390/mi12050490

Figure Lengend Snippet: Glioma-on-chip platforms for photothermal therapy. ( A ) Schematic illustration of the generation of the 3D tumor spheroids. ( B ) Microscope image of the encapsulated cell in the generated droplets (scale bars are 100 μm). ( C ) Fluorescence microscopy images showing the effects of NIR laser treatment on brain tumor spheroids. Live cells were stained with calcein AM (green) while dead cells were stained with ethidium homodimer (red) (scale bars are 100 μm). ( D ) Results of NIR laser irradiation on the cell viability of U87MG brain tumors after being treated with rGO-BPEI-PEG nanocomposites (red bars) and control experiments without the addition of rGO-BPEI-PEG nanocomposites (black bars). ( E ) Correlation of oil flow rate in the junction to the concentration of encapsulated cells in a single droplet . Reproduced with permission from . ( F ) Schematic view of hydrogel microfluidic co-culture device. ( G ) Photothermal therapy of cancer cells. Fluorescent images of cell viability in square-shaped microchambers (green dots represents live and red dots are dead cells) (scale bars are 100 μm) ( H ) Quantitative results of the viability of U87MG and MCF7 cancer cells using CCK-8 assay . Reproduced with permission from .

Article Snippet: As another high-throughput drug combination study by microfluidic systems, poly(ethylene) glycol diacrylate (PEGDA)-based 3D tumor-on-chip model was used to culture U87 human GBM cells to test pitavastatin and irinotecan.

Techniques: Microscopy, Generated, Fluorescence, Staining, Irradiation, Concentration Assay, Co-Culture Assay, CCK-8 Assay

Journal: Micromachines

Article Title: Glioma-on-a-Chip Models

doi: 10.3390/mi12050490

Figure Lengend Snippet: Glioma-on-chips for drug studies. ( A ) 3D bioprinted GBM-on-a-chip between the glass substrates (slides). Bioink I containing BdECM and vascular endothelial cells; Bioink II containing BdECM and GBM cells; and gas permeable silicon ink constructing the ex vivo microfluidic platform . Reproduced with permission from . ( B ) Image of exosomal RNA assay platform. The platform included four different regions for four distinct functions to analyze the cancer exosome . Reproduced with permission from . ( C ) Representative illustration of the fabrication of PEGDA hydrogel-based microchip for drug screening . Reproduced with permission from .

Article Snippet: As another high-throughput drug combination study by microfluidic systems, poly(ethylene) glycol diacrylate (PEGDA)-based 3D tumor-on-chip model was used to culture U87 human GBM cells to test pitavastatin and irinotecan.

Techniques: Ex Vivo, MicroChIP Assay

Journal: Micromachines

Article Title: Glioma-on-a-Chip Models

doi: 10.3390/mi12050490

Figure Lengend Snippet: Requirements of glioma-on-chip platforms and the possible contribution of microfluidic chips [ 53 , 54 , 55 , 56 , 57 , 58 ].

Article Snippet: As another high-throughput drug combination study by microfluidic systems, poly(ethylene) glycol diacrylate (PEGDA)-based 3D tumor-on-chip model was used to culture U87 human GBM cells to test pitavastatin and irinotecan.

Techniques: Permeability, Imaging, Diffusion-based Assay, Migration, Single-cell Analysis, Wound Healing Assay, Cell Culture, High Throughput Screening Assay

Journal: Micromachines

Article Title: Glioma-on-a-Chip Models

doi: 10.3390/mi12050490

Figure Lengend Snippet: Commonly used 3D printing methods for microfluidic chip fabrication and 3D bioprinting.

Article Snippet: As another high-throughput drug combination study by microfluidic systems, poly(ethylene) glycol diacrylate (PEGDA)-based 3D tumor-on-chip model was used to culture U87 human GBM cells to test pitavastatin and irinotecan.

Techniques: Inhibition

Journal: PLoS ONE

Article Title: Three-Dimensional Microfluidic Tri-Culture Model of the Bone Marrow Microenvironment for Study of Acute Lymphoblastic Leukemia

doi: 10.1371/journal.pone.0140506

Figure Lengend Snippet: (A) The microfluidic platform consists of 4 microchannels, each 500 μm in width, 75 μm in height and 2 cm in length. The divergent angles are 30°. Near the inlets and outlet there are perfusion channels. The enlarged boxed area illustrates multiple cell types embedded in a 3-D matrix. (B) Optical image of a microfluidic platform. (C) SEM images of top view (upper panel) and side view (lower panel) of the perfusion channels.

Article Snippet: To study ALL in a model of in vivo relevance, we have developed a 3-D microfluidic tri-culture model in a biomimetic manner.

Techniques: